Re: CE pep analysis

bcdchin@muccmail.missouri.edu
Tue, 10 Feb 98 09:56:50 CST

With CE the concentration is higher than with a RP, because the latter can be
used as a capture of a larger less concentrated sample and concentrated release.
In a nutshell, I would be trying HPLC RP with a C18.


David T. Chin, Director
UMC Protein Core
e-mail: bcdchin@muccmail.missouri.edu
(573)882-2027 (office and messaging)
http://www.missouri.edu/~pc/

To all ABRF's,

I am trying to analyse dipeptides and tripeptides in low concentrations (<<10
mikrogram/ml) with CE.
I have a problem with the stability and reproducibility.

I am using an underivatized silica capillary and
precondition it with NaOH, H2O, H3PO4, H2O and
50 mM Phosphate buffer pH=2,3.
Run voltage=15 kVand run temperature=10 C.
Detection 214 nm.

The peaks in the electropherogram seem to have
different migration time from one analysis to another.
Does anyone think that a change in voltage or
temperature would help to stabilize the method?
Or can any other parameter be changed to improve
the resulted electropherograms?

Thanks in andvance!

Maria Thyboll
Royal Institute of Technology
email: k93_mtl@k.kth.se