Also check journal publications from Fontana's lab.
Note: "Both methionine and the sulfoxide are stable to hydrolysis in
evaculated sealed tubes with hot 3 N p-toluenesulfonic acid" -Fontana
-Lowell H. Ericsson, Dept. of Biochemistry, U. of Washington, Seattle, WA
On Tue, 10 Feb 1998, Lawrence J. Dangott wrote:
> Does anyone have experience in analyzing for methionine sulfoxide? I seem
> to recall that we should use alkaline hydrolysis as the methionine
> sulfoxide will be converted to methione in acid. Any experience or
> protocols for this method you can share? We are using precolumn FMOC/OPA
> derivatization and would appreciate any information before we dive in.
>
> Thanks,
>
> Larry Dangott
>
>
>
>
>
> Larry Dangott, Ph.D.
> Protein Chemistry Laboratory
> Texas A&M University
> College Station, TX 77843-3255
> (409) 845-2965
>
>