______________________________ Reply Separator _________________________________
Subject: Re: The best way to separate glycoproteins
Author: Anthony Yeung <AT_Yeung@FCCC.edu> at SMTPMED
Date: 2/10/98 6:32 PM
If you are to use conA to purify glycoproteins, do pay attention to a well
known conA leaching problem from conA agarose. Crosslinking of the conA
helps, but does not eliminate the leaching.
Tony
At 07:58 AM 2/10/98 EST, ccarrawa@mednet.med.miami.edu wrote:
>
> Lectin-agarose columns are great for this purpose. The appropriate
> lectin must be selected for each glycosylated protein, which can be
> done by screening on blots. Common lectins used for this purpose
> include Con A and wheat germ agglutinin, but others are also used.
>
> Another consideration is release of the bound glycoprotein with the
> appropriate hapten sugar. It is not always best to select the lectin
> with the highest avidity of binding, since it will be more difficult
> to release the glyprotein.
>
> Good luck!
>
>
>______________________________ Reply Separator
_________________________________
>Subject: The best way to separate glycoproteins
>Author: Bao-Shiang Lee <boblee@uic.edu> at SMTPMED
>Date: 2/10/98 7:25 AM
>
>
>Hi, everyone, I need some suggestions in separating glycoproteins from
>non-glycoproteins. Are there columns that can do the job. I would like to
>also separate glycoproteins. Best Wishes. Bob
>
>
>
>
************************************
Dr. Anthony T. Yeung, Ph.D.
Director, Fannie E. Rippel Biotechnology Facility
Member, Institute for Cancer Research
Fox Chase Cancer Center
7701 Burholme Ave. Philadelphia, PA 19111
Voice: 215-728-2488
FAX: 215-728-3647
email: AT_Yeung@FCCC.edu
http://www.fccc.edu/research/labs/yeung/
************************************