Re: AAA of Met(O)

Bernie Mcinerney (Bernie_Mcinerney@bioaust.com.au)
16 Feb 1998 18:03:04 +1100

Reply to: RE>AAA of Met(O)
Larry,
An alternative method of Met(O) analysis is to hydrolyse with 4M methanesulfonic
acid. After hydrolysis neutralise with NaOH (methanesulfonic acid is not volatile)
prior to derivatisation and analysis. Trp can also be determined under these
conditions.
In my hands, using the standard HP AminoQuant (precolumn OPA/FMOC) separation
conditions Met(O) elutes just after Thr and with the Cys-MPA reagent peak as
detected by UV at 338nm. This reagent peak probably will not be a problem if
using fluorescence detection.

Bernie McInerney
Biotech Australia
Roseville NSW 2069
Australia
email: bernie_mcinerney@bioaust.com.au

--------------------------------------
Date: 11/2/98 8:51 AM
To: Bernie Mcinerney
From: Lawrence J. Dangott
Does anyone have experience in analyzing for methionine sulfoxide? I seem
to recall that we should use alkaline hydrolysis as the methionine
sulfoxide will be converted to methione in acid. Any experience or
protocols for this method you can share? We are using precolumn FMOC/OPA
derivatization and would appreciate any information before we dive in.

Thanks,

Larry Dangott

Larry Dangott, Ph.D.
Protein Chemistry Laboratory
Texas A&M University
College Station, TX 77843-3255
(409) 845-2965

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Date: Tue, 10 Feb 1998 09:05:56 -0600
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From: dangott@chemvx.chem.tamu.edu (Lawrence J. Dangott)
Subject: AAA
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