I derivatized peptides (from trypsin and chymotrypsin digestion of protein,
i.e., hemoglobin) with dinitrofluorobenzene in water/dimethyl formamide
mixture. Most formed dinitrophenyl (DNP)-peptides precipitated. After
thiolysis to remove DNP-tag from Cys, His and Tyr, only DNP-NH- left.
However, there were still significant amount of pellets in the solution.
Does anyone know a better solvent to dissolve DNP-peptides? Thanks.
Xiaohui Chen, Ph.D.
Department of Biochemistry
School of Medicine
Case Western Reserve University
2109 Adelbert Road
Cleveland, OH 44106
Phone: 216-368-3250
Fax: 216-368-3419
Email: xhchen@biocserver.cwru.edu