charge heterogeneity

Jason Eglinton (jeglint2@waite.adelaide.edu.au)
Tue, 17 Feb 1998 10:23:41 +1030 (CDT)

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Elliott Nickbarg: "Soundproofing an MS room -Reply"
Previous message: Vivian Morera: "(Fwd) (Fwd) aminoacid analysis"

I have a problem understanding the source of charge heterogeneity in an
enzyme I work with. The preparation is pure as judged by complete peptide
mapping, but exhibits multiple (5 or 6) bands on a native IEF gel. The
sample is reduced prior to IEF, so it is not due to dimer/aggregate
formation. The enzyme has proven resistant to ionisation, so ESI and
MALDI MS have not helped.

Does anyone have examples of charge heterogeneity due to conformational
changes or interaction with carrier ampholytes? I look forward to your
suggestions.

Jason Eglinton ph (08) 8303 7286 fax (08) 8303 7109
Department of Plant Science
Waite Agricultural Research Institute
The University of Adelaide

Next message: Elliott Nickbarg: "Soundproofing an MS room -Reply"
Previous message: Vivian Morera: "(Fwd) (Fwd) aminoacid analysis"