MS - target cleaning etc.
Ulf Hellman (ulf.hellman@licr.uu.se)
Thu, 19 Feb 1998 15:28:57 +0200
Dear ABRF community,
Since about 6 weeks we are in the MALDI-TOF business. Great fun and
impressive sensitivity - a lot of important data has been generated. Sofar
mainly on synthetic and proteolytic peptides, the latter from both
Coomassie- and silver-stained gels.
We have a lot of questions; I'll ask the two most urgent ones:
1. How to clean the stainless steel targets? Our Bruker Biflex is equipped
with the 26 sample circular holder. We have followed various protocols -
none removes absolutely everything. "Ol' history" is becoming annoying!
2. I've learnt that internal calibration gives highest accuracy; the
autodig products of proteolytic enzymes should be ideal. Anyone knows if
the Promega modified trypsin, with its methylated Lys side chains, produces
any useful "calibration standards"? Same for Achromobacter lyticus LysC?
Thanks /Ulf
******************************************************
Ulf Hellman, Ph. D.
Ludwig Institute for Cancer Research :
Box 595
S-751 24 Uppsala
Sweden
Phone +46 18 160423
Fax +46 18 160420
E-mail ulf.hellman@licr.uu.se
Lab's home page: http://www.licr.uu.se