I want to do a CNBr-cleavage of a hydrophobic 140 kD protein, which is not
soluble in aqueous solution. So I plan to add some acetonitrile to the 70%
aqueous formic acid during reaction.
Is this advisable or are there other (better) ways of doing a Br CN
cleavage with an water insoluble protein?
Thanks in advance for taking your time!
Henriette
Dr. Henriette A. Remmer
Director of Protein Services
University of IIllinois
311 Noyes Laboratory Box 62-1
505 S Mathews Ave
Urbana, IL 61801
phone: (217)333-4695
fax: (217)244-1142
email: remmer@staff.uiuc.edu