We are having the infamous "fast fade" problem and in searching the
Automated DNA Sequencing WWW Page searchable index (many thanks to those
responsible for that fine page) I find varied and contradictory bits of
info.
Listed cures included: specific detergents, strong NaOH washes, strong HCl
washes, scalding hot water washes, using a dishwasher, voodoo and more.
My request is for anyone who thinks she or he really has a handle on this
problem, i.e. has managed to beat this problem for at least 6 months, to
please respond with your cure so I can make a better plan of attack.
PS - we have had some modest success with Multi-terge, at least we can read
the bases in most cases within the fade region, but still hope that there
is something better out there.
PPS - we did not have this problem 'till Big Dye came to town and we are
only doing 1:2 dilutions. Coincidence or not? The archives show fade
problems dating long before BD's but I'm wondering if larger dilutions of
DBs help the fade problem. Any chance of a lot problem here or is this a
permanent condition for everybody. We just saw the fade on a brand new set
of plates!
Thanks to all in advance,
Dick Cook