Gary, I made up fresh A and the problem did not go away. The lysine in the
standard runs fine. I'm thinking there may be an air leak in the sequencer.
Charles
_______________________________________________________________________________
Subject: Re: Prot Seq: Low Lysine
From: Gary Hathaway <hathaway@cco.caltech.edu> at INTERNET
Date: 02/23/1998 21:08
At 5:43 AM -0700 2/23/98, CHARLES MCNEMAR wrote:
>When I run B-lactoglobulin on the 477 and 494, I get about 95% repetitive
>yield
>on both instruments. On the 494, the K-8 is about 30% of the M-7 and G-9.
>Does anyone have any experience solving this problem?
>
>Charles,
You might try switching A buffer? If the problem follows the old buffer,
you have it. Old A buffer will result in a decrease in PTH-amino acid
yield. Lysine seems particularly susceptible. This may be due to free
radical buildup.
regards
--------------------------------------------------
Gary M. Hathaway, Director
PPMAL - Protein/Peptide Micro Analytical Laboratory
California Institute of Technology
139-74, Division of Biology
Pasadena, CA 91125
http://www.cco.caltech.edu/~ppmal
email Gary: hathaway@caltech.edu
email facility: ppmal@caltech.edu
phone: lab (818) 395-6388 or office (818) 395-2769
FAX (818) 449-3414
-------------------------------------------------
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Subject: Re: Prot Seq: Low Lysine
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