Re: another CNBr-cleavage question

Gautam Sarath (gsarath@unlinfo.unl.edu)
Tue, 24 Feb 1998 08:51:00 -0600 (CST)

> We are synthesizing a peptide ARRTRRPMLRAVRIIAE . The Arg side
>chain group is [Pfb]. Has anyone had any experience using Fmoc chemistry
>for this side chain group, and what cleavage conditions were used?
>
>-----------------------------------------------------------------------------
>Mark Morris email: pacl@mgrp.bgsm.edu
>Biochemistry Department Phone: 336-716-2581
>Protein Analysis Core Laboratory Fax: 336-716-7200
>Bowman Gray School of Medicine
>Wake Forest University
>Medical Center Blvd.
>WInston-Salem, NC 27157
>-----------------------------------------------------------------------------

Mark: Use Reagent K, 3 to 4 hours ought to do it. Precipitate into cold
t-butylmethylether. Coupling with HATU/DIPEA. good luck, Gautam Sarath

Gautam Sarath
N-226, Beadle center
Protein Core Facility
Department of Biochemistry
University of Nebraska-Lincoln
Lincoln, NE 68588-0664
Phone: 402-472-2928
FAX: 402-472-7842