Re: another CNBr-cleavage question

David A. Schooley (schooley@unr.edu)
Tue, 24 Feb 1998 08:26:06 -0800

Mark-
Yes, we use it routinely. Use reagent K (Int. J. Peptide Protein
Res., 36, 255-266 (1990). For more information on synthesis and
deprotection of difficult peptides, see David King's article in 1996 issue
#4 of ABRF news, available on line at
http://www.medstv.unimelb.edu.au/ABRFNews/1996/December1996/Long_PepSyn.html

> We are synthesizing a peptide ARRTRRPMLRAVRIIAE . The Arg side
>chain group is [Pfb]. Has anyone had any experience using Fmoc chemistry
>for this side chain group, and what cleavage conditions were used?

David

David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@med.unr.edu
tel: (702) 784-4136; fax (702) 784-1419