dilution buffer for BigDyes

Harold G. Hills (hhills@iastate.edu)
Tue, 24 Feb 1998 11:34:39 -0500

I have just been talking with our PE-ABD sales rep who says that yes, the
5X dilution buffer should be diluted in half and used at 2.5X to replace
the DB premix.

When using 1 ul of the BigDye premix, the results are clearly better with
2.5X than with 5X.

Check this out.

Hal

Harold G. Hills, Ph.D. DNA Sequencing Specialist 515 294-9585
1184 Molecular Biology Building FAX 515 294-1597
Iowa State University hhills@iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF For info about facility.