I have synthesized a peptide with the sequence CDGSVWAQSESFPELK. The HPLC
shows two major peaks adjacent to each other with the major peak being a
dehydrated (-18) species of the peptide as shown by mass spec. I suspect
pyroglu. formation, but I'm not sure where. Can anyone comment on what
might have happened here and what I can do to prevent this if I need to
resynthesize??
Thanks in advance!
Mary
Mary Bower
Instrument Specialist
Purdue University
Department of Biochemistry
West Lafayette, IN 47907
Phone (765)494-6540
Fax (765)494-7897
email; Bower@Biochem.purdue.edu