Re: Peptide Synthesis

alessandro tossi (tossi@univ.trieste.it)
Thu, 26 Feb 1998 11:08:37 +0100

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Mary Bower wrote:
>
> Hello everyone,
>
> I have synthesized a peptide with the sequence CDGSVWAQSESFPELK. The HPLC
> shows two major peaks adjacent to each other with the major peak being a
> dehydrated (-18) species of the peptide as shown by mass spec. I suspect
> pyroglu. formation, but I'm not sure where. Can anyone comment on what
> might have happened here and what I can do to prevent this if I need to
> resynthesize??
>
> Thanks in advance!
>
> Mary

Mary, did you use DBU in your Fmoc cleavage solution? If so, you may
have dehydrated the Asp.

Bye, Alex

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