The discussion going on regarding the Edman degradation chemistry
and MS is interesting, particularly the real world scenerio expressed by
Joe.
Anyone at the Core laboratories performing C-terminal ladder
sequencing using a Mass Spectrometer? We have a couple of samples
at 0.3 - 0.5 pmole level, if any MS laboratory can identify 4-5 C-terminal
residues. What will be your charges.
Thanks in advance
Satya Yadav, Ph.D.
Director
Molecular Biotechnology Core
Lerner Research Institute
The Cleveland Clinic Foundation
Tel 216-445-7095