</fontfamily><fontfamily><param>New_York</param>Congratulations to Jim
on surviving the MS tsunami....most all of it seemed of good nature;
all of it was informative...I was afraid you would hide low for a few
years, Jim. </fontfamily><fontfamily><param>Times</param>
</fontfamily><fontfamily><param>New_York</param>I am a newbie to ESI MS
and when I read Jim's posting my first thought was "why IS running
Edman sequencers such a pain and does it have to be so?" I was thinking
that one could spend more time learning MS if one had to worry less
about the Edman machines.
</fontfamily><fontfamily><param>Times</param>
</fontfamily><fontfamily><param>New_York</param>I have been very happy
that prot seq and pep synth etc. has leveled off somewhat, as far as
new technology goes, over the past few years so I can concentrate on MS
and other things but I think it would be nice if ABRF put together a
compendium of prot seq notes so everyone could profit ...raise the tide
and lift all boats so to speak.....so that all the great techniques
could be hopefully flushed out....and the matter of maintaining a high
sensitivity protein sequencer be put to rest for a few years.... i.e.
get everyone to put in their ca. 5 or whatever favorite Edman tricks
(trick = not in manual or user bulletin) to running a protein
sequencer. Appoint an edit team and give everyone who contributes
credit. Something along the lines of the ABRF non-standard sequencer
peaks and non-standard PTC amino acid peaks information handouts. They
were super.</fontfamily><fontfamily><param>Times</param>
</fontfamily><fontfamily><param>New_York</param>Is there critical mass
for this or is Edman old hat for everyone now?
Dick Cook</fontfamily>