PHOSPHOPEPTIDE SEQUENCING

Robert J. Donnelly (donnelly@umdnj.edu)
Mon, 2 Mar 1998 18:21:04 -0500

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I have a customer who is looking to identify the specific amino acid in =
a 25 amino acid peptide that has been phosphorylated in an in vitro =
phosphorylation reaction. Is there a method available to protect the =
phosphorylated amino acid (in this case serine) in order to identify the =
specific residue. This peptide contains several potential =
phosphorylation sites. Typically the peptide is presented to me on a =
PVDF membrane as the two forms are separable on a gel. =20
Robert J. Donnelly, Ph.D.
Director, Molecular Resource Facility
UMDNJ-New Jersey Medical School
MSB C506
185 South Orange Avenue
Newark, NJ 07103
ph: 973-972-2625
fax: 973-972-2566

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I have a customer who is looking to = identify the=20 specific amino acid in a 25 amino acid peptide that has been = phosphorylated in=20 an in vitro phosphorylation reaction.  Is there a method available = to=20 protect the phosphorylated amino acid (in this case serine) in order to = identify=20 the specific residue.  This peptide contains several potential=20 phosphorylation sites. Typically the peptide is presented to me on a = PVDF=20 membrane as the two forms are separable on a gel. 
Robert J. Donnelly, = Ph.D.
Director, Molecular=20 Resource Facility
UMDNJ-New Jersey Medical School
MSB C506
185 = South=20 Orange Avenue
Newark, NJ 07103
ph: 973-972-2625
fax:=20 973-972-2566
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