cellular contents separation protocol

Winnell Newman (Winnell@ncsu.edu)
Tue, 3 Mar 1998 13:29:37 +0700

Dear all on ABRF:

Can anyone give me some leads for protocols to isolate SIMULTANEOUSLY DNA,
RNA and protein from cells...probably yeast , Pls?

I would like to use this in an application where I am "feeding" isotope
oxygen (heavy water) to yeast, then harvest the DNA, RNA and Protein for
those contents that successfully incorporated the isotope ....

Any clues will be appreciated.

Sincerely,

Winnell Newman

Winnell H. Newman Director, Nuclei Acids Facility
919-515-3463 office North Carolina State University
919-515-3355 fax Dept of Genetics Box 7614 (mail)
winnell@ncsu.edu 3513 Gardner Hall (shipping)
Raleigh, NC 27695