MS/MS of peptides with disulfide bridges

Kevin Wheeler (kevinwhe@ix.netcom.com)
Tue, 03 Mar 1998 10:33:15 -0800

I would like to tap the groups experience on MS/MS of peptides with
disulfide bridges. Recently, we were brought a synthetic peptide with 2
disulfide bridges in a 15-20-mer peptide. My first suggestion was to
reduce/alkylate the sample, but I am wondering if anyone out there
routinely gleans data from non-reduced forms of disulfide bridged
peptides?

On a similar theme, what approaches are taken for the interpretation of
MS/MS of cyclic peptides?

Thanks in advance.

-- 
Kevin Wheeler
Sales Representative
ThermoQuest Corporation
PO Box 19811
Seattle, WA 98109-6811
Office: 206-583-0923
wheeler@finnigan.com