Re: cellular contents separation protocol

Michael N. Horst, Ph.D. (horst_mn@Mercer.EDU)
Wed, 04 Mar 1998 07:59:04 -0500

Check out Tri-Reagent from Sigma.

Winnell Newman wrote:

> Dear all on ABRF:
>
> Can anyone give me some leads for protocols to isolate SIMULTANEOUSLY DNA,
> RNA and protein from cells...probably yeast , Pls?
>
> I would like to use this in an application where I am "feeding" isotope
> oxygen (heavy water) to yeast, then harvest the DNA, RNA and Protein for
> those contents that successfully incorporated the isotope ....
>
> Any clues will be appreciated.
>
> Sincerely,
>
> Winnell Newman
>
> Winnell H. Newman Director, Nuclei Acids Facility
> 919-515-3463 office North Carolina State University
> 919-515-3355 fax Dept of Genetics Box 7614 (mail)
> winnell@ncsu.edu 3513 Gardner Hall (shipping)
> Raleigh, NC 27695