Hi All,
I am currently developing a protocol for amino acid analysis from PVDF
blots. However, I am finding human keratin, and collagen to be a major
contamination problem, despite the use of gloves etc.
Does anyone else have any experience of this? My ideal solution would
be some form of wash which would remove the surface contaminants, whilst
leaving the electroblotted protein untouched. This would be performed
before hydrolysis. As I do not prepare my own samples, a change in the
blotting techniques would not really be practical, so I'm looking for
damage limitation!
Thanks in Advance
Russell