We have a narrow hot water bath beside our preparative HPLC and just dunk
the prep. column in the water and set the temperature to whatever is
necessary. Its cheaper than getting a column heater and makes a nice backup
to the main hot water bath. We heat the sample in it too just prior to
injection.
Dick Cook
>Hello
>I have been told that heating RP columns reduces the amount of peptide
>one loses during purification. This is a serious problem for us, as we
>deal with quite basic peptides, and I understand that incomplete capping
>of the silica matrix could lead to retention. How can we efficiently
>thermostat a preparative column (3 cm diameter, 30 cm length. I was
>wondering if thermal tape could be the answer. We have a local company
>that make thermostated holders for the solvent reservoirs.
>
>Thanks, Alex
>
>======================================================================
>Dr. Alessandro Tossi
>Antiinfective peptides group
>Dept. of Biochemistry, Biophysics and Macromolecular Chemistry
>University of Trieste
>Phone -- 39 40 6763673; Fax -- 39 40 6763691
>email: tossi@univ.trieste.it
> tossi@icgeb.trieste.it
>http://www.univ.trieste.it:80/~nirdbbcm/antimic.html
>=====================================================================