In gel digests on stored, dried gels

Kenneth Williams (Kenneth.Williams@yale.edu)
Fri, 06 Mar 1998 09:43:31 -0500

Susanne,
We cut out the dried, Coomassie Blue stained spots and then rehydrate each
in an eppendorf tube for about 30 minutes in water. We then remove the
plastic backing from the tube with clean forceps prior to carrying out our
usual washing steps. Although we have not compared storage conditions,
some of our best in gel digests have come from dried 2D gels that were
stored for weeks-months taped into notebooks kept on shelves. Good luck
with your digests.

Ken Williams

______________

At 11:15 AM 3/6/98 +0100, you wrote:
>I really enjoy the discussion in-gel and/or on-membrane digest. In
>relation to this I want to ask for a procedure to analyse by in-gel
>digestion (e.g. by trypsin) of 2D gels, which have been stored dry on
>plastic backing/support (Pharmacia ready made gels). About a year ago
>experiences with 2D gels dried between two sheets of cellophane in
>relation to in-gel digest were discussed here. However, I can not
>remember a procedure for re-swelling the gels.
>
>With the ready-made gels you have not a protective layer on both sides
>of the gel. By the way what is the best method of storage 2D gels on
>plastic support that one might to keep for an extended period of time
>for in-gel digestion?
>
>
>Thanks for your experience,
>
>
>Susanne
>
>-----------------------------------------------------------
>Susanne Jacobsen
>Department of Biochemistry and Nutrition, Building 224
>Technical University of Denmark, DK-2800 Lyngby, Denmark
>
>Telephone: (+45) 45 25 27 41, Telefax: (+45) 45 88 63 07
>
>E-mail: suja@mimer.be.dtu.dk or suja@biobase.dk
>------------------------------------------------------------
>
>