Halfterm and DNA sequencing buffers

Richard T. Pon (rtpon@acs.ucalgary.ca)
Tue, 10 Mar 1998 09:39:28 -0700 (MST)

Hi
Our facility has been using the "Half-term" buffer sold by Gen-Pak
in all of our FS sequencing reactions (4 ul rxn mix + 4 ul half-term
buffer) for some time now with good results. Once we have used up our
existing FS kits, we plan to switch to dRhod chemistry. However, I suspect
that the "Half-term" buffer is nothing more than the regular buffer ABI
uses in their sequencing kits.
Does anyone know if this is true? More importantly, does anyone
know if it is possible to purchase sequencing buffer for FS or dRhod kits
separately? Will the Big-Dye buffer from ABI serve the same purpose when
used with FS or dRhod kits?

Thanks

Richard T. Pon, Director E-mail: RTPon@acs.ucalgary.ca
University Core DNA Services Phone: (403) 220-4277
University Of Calgary Fax: (403) 283-4907
Please direct DNA synthesis or sequencing orders to DNALAB@acs.ucalgary.ca