Re: Recovering proteins from SDS gels

Frank_Masiarz@cc.chiron.com
Wed, 11 Mar 1998 13:42:59 -0800

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Hi..............

Please refer to:

Proc. Natl. Acad. Sci. USA, Volume 90, 1927-1931 (1993)

The paper contains a description of a method (with references) upon
which I have relied for many years: electro-elution. The elution cell
described in the paper may still be available from the California
Institute of Technology.

The acetone precipitation method removes the vast majority of SDS and
Coomassie blue, although, in some cases, there have been signs of SDS
retention in the mass spectra.

I have had no problems dealing with 0.5-1.0 microgram of protein
purified by 1D SDS-PAGE or 2D SDS-PAGE.

Good Luck !!

Frank R. Masiarz
Director
Protein Structure Analysis Group

Chiron Corporation
4560 Horton Street
Mailstop B-367
Emeryville, CA 94608-2916

(510) 923-2827 - FAX (510) 655-8453

______________________________ Reply Separator _________________________________
Subject: Recovering proteins from SDS gels
Author: Bryan Dunbar <b.dunbar@abdn.ac.uk> at SMTP
Date: 3/10/98 11:15 AM

Hello folks,
can anyone suggest "proven" protocols for recovering
proteins/peptides from 2-D gels in a form conducive for MS. There are
loads of methods in the literature, I'm sure - but I would appreciate
hands-on experiences. Many thanks in advance.
Bryan.

-- 
Bryan Dunbar,
Protein Facility,
Room WT27,
Department of Molecular & Cell Biology, 
University of Aberdeen,
Polwarth Building,
Foresterhill,
Aberdeen,
SCOTLAND.
AB25 2ZD.
TEL: 01224-273103
FAX: 01224-273104
e-mail; b.dunbar@abdn.ac.uk

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