AAA Protein mass
Susana Linskens (linskens@qb.ffyb.uba.ar)
Thu, 12 Mar 1998 10:46:28 -0300
Dear Jos:
I also feel not safe with this problem. What I do, very often, is to
run Hydrolysis Test Peptide, with Nor-Leucina incorporated in the same solution.
I do take care in loading the cleanest as possible, doing that with an old
glass micropipette of 10 microliters, always with the same and washing once
with TFA/Acetonitrile/water solution, adding that to the frit. Having a
"good "stantard calibration ( that means 10 Pierce samples averaged ), I do
an average of , let's say, 6-8 Hydrolysis Test peptides, normalizing with
the recovery of Nor-Leucina . In the same way, I process several B-LG samples
( B-LG from ABI ). The recovery I am having is around 60 %. When I need to
have a very good mass determination of a sample, I process it in the same way
and I correct the result in basis of this 60 %.
I do not realize any problem with the solubility of the Test Peptide.
But, again, I am always worried about that.
I do not know of another standard to check this. Of course, I will be aware
if somebody suggests something useful for this subject.
Good luck
Susana
Susana Linskens
J.M.Gutierrez 2600- 14 A
1425 Buenos Aires - ARGENTINA
linskens@qb.ffyb.uba.ar
FAX: University:54-1-963-9677
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