our laboratory has bought a Kompact MALDI III machine
three months ago, so we are still very greenhorn to MALDI. We are quite
succesfull with low mass compounds and oligomers, but totally unsuccesfull
with proteins and NAs - e.g. BSA, we have tried all possible matrices (from
sinapinnic till TRIS), all to us known methods of sample preparations, but
per ten sample spots we got only one reasonable result, and it seems to be
not good: 67720 Da for BSA.
Where is the mistake we did and do? Can someone help us, please?
Jan Havlis,
Dept. of Analyt. Chem.
Fac. Sci. Masaryk University (URL http://www.sci.muni.cz/)
Brno, Czech Republic
Hojudijaca onale hijemor,
Lakantunok atrojum hijor Gods will be on your side
ce atrojumhi taxmele only if you go in their direction
ge pelije ragin ifrab. and thereto wisdom is needed.
Jan Havlis
jdqh@chemi.muni.cz
http://www.chemi.muni.cz/~jdqh/