AAA-tryp

Sandra Smith (sandie@mail.utexas.edu)
Fri, 13 Mar 1998 16:41:41 -0500 (EST)

Dear ABRFers,
I need to analyse 8-10 whole protein cell extracts for tryp. In the past
IVe used dodecanethiol with success but rather than work out all those
conditions again for my rather testy autohydrolyser, I thought I'd do
manual hydrolsis. My question is: Can I do methanesulfonic acid hyd vapor
phase? Or could I do a manual hydrolysis using dodecanethiol? What
conditions? Thanks in advance.
Sandie

Sandie Smith
Protein Microanalysis Facility,
Institute for Cellular and Molecular Biology
University of Texas, Austin, Texas 78712
Email: sandie@mail.utexas.edu
Phone: 512 471-3741, Fax: 512 471-9651