I would like to cut bands out of an SDS gels and run them on a HPLC
attached to a ESIMS. If your facility does this, I would like to know how
sensitivity your set up is. Specifically, if I have recovered 5 pmols of a
protein or protein digest from the gel, can you see these
peptides/proteins in the MS given your current HPLC/MS configuration?
Can you see 1 , 0.5 or 0.2 pmol? How low can you go before your
signal-to-noise ratio drops below 10 (or what ever ratio you feel
confident will provide reliable MW determinations)?
If you want to send your results directly to me, I will tally all the
responses into one table and post the results.
Thanks for your help.
Tom Haas
haast@cesmtp.ccf.org
Molecular Cardiology
Cleveland Clinic Foundation
9500 Euclid Ave
Cleveland, OH 44122
"Home of the Rock-and-Roll Hall of Fame and the Cleveland Browns 99"