Yes, I always clean the slides with the cleaning cycle (cycle #2 for me)
after the run is finish but, as you know, this may be not enough especially
if you have loaded a large amount of protein or samples contaminated by
salt.
What I suggest to you is to run once a week a derivatizer cycle for all the
slides whithout any loading.
This was a suggestion that D.Mazzocout ( ABI-France technical assistance )
gave to me and seems to work; you should also control very carefully the
level of the glass fiber in the frits because if it is not level could lead
to variations during extraction of the derivatizer sample.
For new slides first spot them with 40mcl of 0.025% K3EDTA and then run the
derivatizer cycle.
I would avoid to pyrolys the slides because the high temperature can really
change the structure of the glass fiber in the frits.
Ciao
Roberta
E-mail: roberta.napoleoni@serono.com
---------------------------------------------------------------------------
---------------------------------------------------------------------------
----------------------------
JGHOOGER@am.pnu.com on 13/03/98 19.41.58
To: Roberta Napoleoni/IT_ARD01/SERONO
cc:
bcc:
Del. date: 13/03/98 20.37.57
Ref. No: /
Subject: Re: AAA
Roberta,
When you use manual hydrolysis, do you clean the slides after the samples
have been derivatized and analyzed? If so, do you clean them manually or do
you use the hydrolyzer head?
Thank you for any information you can provide on how you handle the slide
cleaning issue.
John Hoogerheide
Analytical Methods & Services
Pharmacia & Upjohn
Kalamazoo, MI USA
jghooger@am.pnu.com