post synthesis peptide workup
Michael Jobling (m.jobling@pgrad.unimelb.edu.au)
Mon, 23 Mar 1998 15:24:13 +1000 (EST)
Hello everybody.
I am looking for the most suitable method to desalt my peptides after
cleavage, usually as a result of removing a formyl group from tyrptophan (I
use Boc-chemistry). I was hoping a size-exclusion chromatography gel could
desalt, remove any trace amounts of scavenger left over from HF cleavage
and maybe even remove major deletion products. The question is does anybody
have any suggestions to which gel I should use (suppliers name would be
appreciated too).
Also when removing formyl groups prior to cleavage should it be done before
or after removing a Dnp protecting group?
Regards
________________________________________________________________________________
Michael Jobling Department of Pathology
Dept. of Pathology Ph: +61 3 9344 5874 Fax: +61 3 9344
4004
The University of Melbourne School of Chemistry
Parkville Ph: +61 3 9344 7272 Fax: +61 3 9347
5180
Australia 3052 email: m.jobling@pgrad.unimelb.edu.au
________________________________________________________________________________