RE: Mass analysis of uncharged peptide
Manfred Raida (Manfred_Raida@compuserve.com)
Thu, 26 Mar 1998 02:58:16 -0500
Hello Jim,
I once had a similar problem, a peptide which did not contain any group
which could be possitively charged and in addition a phosphorylation. I
increased the formic acid in my ESI solvent to 1 % (50 % ACN or MeOH in
water) and it worked fine. I also tried a negative ESI under basic
conditions...nothing. Just try it.
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Manfred Raida
Niedersaechsisches Institut fuer Peptid-Forschung
(IPF)
Dep. of Analytical Peptide-Chemistry
Feodor-Lynen Strasse 31
D-30625 Hannover
Germany
E-Mail Manfred_Raida@compuserve.com
Tel.: +49 511 5466 210
Fax: +49 511 5466 102
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