cysteines

john holt (jholt@voicenet.com)
Thu, 26 Mar 1998 19:22:58 -0500 (EST)

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Does anyone have an idea how I might keep cysteines reversibly reduced
during a purification using reverse phase HPLC under the usual 0.1% TFA
conditions?? I know traditional alkylation works, and allows me to achieve
the purification, and I know reduction and hoping
-that-reoxidation-won't-occur-upon-lowering-the-pH and proceeding, doesn't.

John Holt

Pasteur Merieux Connaught

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