RE: P2D: Separation Power of 2D Gels

Alex Apffel (alex_apffel@hpl.hp.com)
Thu, 26 Mar 1998 15:44:25 -0800

Hi Tony,
Yes I did see Greg Opiteck's talk and what has sparked my curiosity is
whether his results are limited by the specific hardware incarnation or more
basic physics and thermodynamics of the separation mechanisms. To evaluate
that I'd like to be able to understand what the separation power of 2D gels
is controlled by. My experience so far is that this is spoken of mostly
from a empirical or folk-lore point of view as "100's of thousands of
spots". If I had a more basic understanding, I could compare it to HPLC
modes in which I can calculate separation power.

Alex Apffel, Ph.D.
Biomeasurements Group
Hewlett-Packard Laboratories
3500 Deer Creek Road
Palo Alto, CA 94304, USA
Tel 650-857-6090
Fax 650-852-8502

> -----Original Message-----
> From: Anthony Yeung [mailto:AT_Yeung@fccc.edu]
> Sent: Thursday, March 26, 1998 3:18 PM
> To: Alex Apffel
> Subject: Re: P2D: Separation Power of 2D Gels
>
>
> Alex:
>
> Were you at the ABRF meeting? There was this wonderful talk from Gregory
> J. Opiteck of UNC on the use of 2D HPLC coupled to MS. He said he is no
> where close to the power of 40 cm 2D gels. He is resolving only 170 peaks
> after MS ID of the 2D HPLC resolution.
>
> Tony
>
> At 08:06 AM 3/26/98 -0800, you wrote:
> >I'm trying to compare HPLC Separations with 2D PAGE Gels in Proteomic
> >Applications. Can anyone refer me to a reference or discussion of
ultimate
> >and practical separating power and peak capacity of 2D Gels?
> >
> >Alex Apffel, Ph.D.
> >Biomeasurements Group
> >Hewlett-Packard Laboratories
> >3500 Deer Creek Road
> >Palo Alto, CA 94304, USA
> >Tel 650-857-6090
> >Fax 650-852-8502
> >
> >
> >
>
> ************************************
> Dr. Anthony T. Yeung, Ph.D.
> Director, Fannie E. Rippel Biotechnology Facility
> Member, Institute for Cancer Research
> Fox Chase Cancer Center
> 7701 Burholme Ave. Philadelphia, PA 19111
> Voice: 215-728-2488
> FAX: 215-728-3647
> email: AT_Yeung@FCCC.edu
> http://www.fccc.edu/research/labs/yeung/
> ************************************
>