At 07:22 PM 3/26/1998 -0500, john holt wrote:
>Does anyone have an idea how I might keep cysteines reversibly reduced
>during a purification using reverse phase HPLC under the usual 0.1% TFA
>conditions?? I know traditional alkylation works, and allows me to achieve
>the purification, and I know reduction and hoping
>-that-reoxidation-won't-occur-upon-lowering-the-pH and proceeding, doesn't.
>
>John Holt
>
>Pasteur Merieux Connaught
>
>