This forum has been so helpful in the past, I wish to spring two more
questions regarding peptide synthesis:
1. A client wishes to couple his active azide-compound to a completed
peptide which is still on-resin. The Fmoc is the only group removed, the
peptidyl- HMP resin (ABI) is dried and in a flask, ready to go.
Apparently, the azide can only be solubilized effectively in neat DMSO. I
wonder about the ability of DMSO to effectively swell the resin - if anyone
can enlighten me I would be grateful.
2. Another problem: I have done a little poking around, but have come up
empty-handed with regard to protocol for producing p-nitroaniline amides at
the COOH-terminus of peptides (pNA peptides). This seems to be used in
proteolytic assays, where the release of the p-nitroaniline can be followed
conveniently using visible wavelengths. Anyone able to point the way?
Thanks for any help,
Alan
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Alan Mahrenholz, Ph.D.
Dept. Biochemistry
1153 S. University St.
Purdue Unversity
West Lafayette, IN 47907-1153
voice: (765) 494-6383
lab: 494-6540
fax (765) 494-7897