People at a company I used to work a few years back did extensive work on
carbohydrate profiling and quantitation using "Fluorophore Assisted
Carbohydrate Electrophoresis" (FACE), wich was pretty new and quite
untested then. As I recall, they showed, after lots of careful experiments
and comparisons with other methods that the technique works very well,
reliably, and reporducibly, and could be used as a QC method. I will not
presume to speak for them at this point, perhaps if someone is reading this
might respond directly. At my previous job I bought an instrument (which
is a gel scanner, really, with a PC and a "kit" consisting of a gel
apparatus and a bunch of enzymes) for my lab and used it (not very much)
with good results.
You might want to check the web site of Glyko (www.glyko.com), a company
which markets the equipment and provides technical assistance. That's one
company I am familiar with, there are probably others.
Ioannis Papayannopoulos
CytoMed, Inc.
Cambridge, MA
At 10:06 AM 3/31/98 -0500, Kelly S Cass-Samodral wrote:
>Hello ABRF members,
>I was asked today what is the method of choice for the quantitation of
>glycoforms on a glycoprotein? Is this being done routinely, ie QC? Are
there
>other ways to monitor the same information?
>All answers accepted. Thank you in advance for your time.
>Kelly Cass-Samodral
>KSCS@lilly.com
>
>
>