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A recent VYDAC newsletter has more information on TFA w/ reverse phase;
it is also free and available on request.
-- *************************************************** Christopher Lapsey Technical Service Western Analytical SF Office 800-241-5145 (415-333-5988) fax 415-333-5989 http://www.WesternAnalytical.com hplc@WesternAnalytical.com ***************************************************--------------56C09E1E1D Content-Type: message/rfc822 Content-Transfer-Encoding: 7bit Content-Disposition: inline
Return-Path: <abrf-request@aecom.yu.edu> Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.4]) by mail.netwiz.net (8.8.5/8.8.4) with ESMTP id OAA11307 for <christopher@WesternAnalytical.com>; Tue, 31 Mar 1998 14:40:29 -0800 Received: (from daemon@localhost) by post.aecom.yu.edu (8.8.8/8.8.8) id LAA25528 for abrf-out; Tue, 31 Mar 1998 11:26:15 -0500 (EST) Received: from UABDPO.DPO.UAB.EDU (UABDPO.dpo.uab.edu [138.26.1.10]) by post.aecom.yu.edu (8.8.8/8.8.8) with SMTP id LAA25521 for <abrf@aecom.yu.edu>; Tue, 31 Mar 1998 11:26:12 -0500 (EST) Received: from Heath.uab.edu by UABDPO.DPO.UAB.EDU (IBM VM SMTP V2R2) with TCP; Tue, 31 Mar 98 10:26:56 CST Message-ID: <35211943.35CE@uab.edu> Date: Tue, 31 Mar 1998 10:26:43 -0600 From: Matteo Villain <villain@uab.edu> Reply-To: Villain@uab.edu Organization: University of Alabama, Birmingham X-Mailer: Mozilla 3.0 (Win95; I) MIME-Version: 1.0 Old-To: chris halkides <halkidesc@UNCWIL.EDU>, abrf@aecom.yu.edu Subject: Re: misc References: <l031107ffb14603f8683e@[152.20.32.41]> Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit To: Recipients of ABRF List <abrf@aecom.yu.edu> Sender: Association of Biomolecular Resource Facilities <abrf-request@aecom.yu.edu> Precedence: bulk Errors-To: abrf-request@aecom.yu.edu
chris halkides wrote: > > Hello Everyone: > > We are analyzing proteins and peptides by HPLC. I am interested in > the stabilities of TFA in water and TFA in acetonitrile/water. Does it > matter if the atmosphere above the solvent bottle is air or nitrogen? > > Thanks! > > Chris Halkides > halkidesc@uncwil.edu > > Christopher Halkides > Dept. of Chemistry, UNCW > 601 S. College Road > Wilmington, NC 28403-3297 It does not matter, but if you are going to use it at low wavelength, (for peptide ideal is 215-220) you should degass with Helium. There is a very nice little book from Vydac about peptide analysis and purification by RP_HPLC, and usually they give it for free. It will tell you a couple of trick, you should get it.
-- Dr. Matteo Villain Phone + 205 934 3032 Research Associate Fax + 205 934 1446 Dep. Physiology and Biophysics University of Alabama at Birmingham 893 BHSB 1918 University Blvd., Birmingham, Al ,35294 USA--------------56C09E1E1D--