Re: Ethylenimine

Gregory Grant (ggrant@pharmdec.wustl.edu)
Wed, 1 Apr 1998 21:28:36 -0600

>Does anybody know where I can buy, beg, or steal this compound. It is
>very toxic, has not been used much so most distributors have discontinued
>carrying it. It can be used to alkylate cysteine which then can be
>cleaved by trypsin or lys-C.
>
>Ref. Methods in Enzymology Vol 11 pg 315-317 (1967) Article on
>S-aminoethylation by David Cole.
>
>-Lowell H. Ericsson, Dept. of Biochemistry, U. of Washington, Seattle, WA
> phone 206-543-5320 FAX 206-685-1792

Lowell,

Pierce sells a less toxic alternative for this purpose. Its called
aminoethyl-8 reagent. (#23010). I've not used it though.

Gregory A. Grant
ggrant@pharmdec.wustl.edu