We've used the 2-bromo ethylamine procedure to generate it in situ.
Sigma sells it and we were able to scale down the original procedure
(Okazaki et al. Anal Biochem 149, 516-520, 1985) to current pmole levels
of sample. We got very nice tryptic digestion at the aminoethylated
cysteines.
Mike
Michael F. Rohde, Ph.D.
Director, Protein Structure
Amgen, Inc. 14-2-E
1840 DeHavilland Dr.
Thousand Oaks, CA 91320
805-447-3091
805-499-7464 (fax)
mrohde@amgen.com
> ----------
> From: L. Ericsson
> Sent: Tuesday, March 31, 1998 3:15 PM
> To: Recipients of ABRF List
> Subject: Ethylenimine
>
>
> Does anybody know where I can buy, beg, or steal this compound. It is
> very toxic, has not been used much so most distributors have
> discontinued
> carrying it. It can be used to alkylate cysteine which then can be
> cleaved by trypsin or lys-C.
>
> Ref. Methods in Enzymology Vol 11 pg 315-317 (1967) Article on
> S-aminoethylation by David Cole.
>
> -Lowell H. Ericsson, Dept. of Biochemistry, U. of Washington, Seattle,
> WA
> phone 206-543-5320 FAX 206-685-1792
>
>
>