For the synthesis of pNA peptides, you might want to check out the
following references:
1. Tuppy, H. et al. (1962) Z. Physiol. Chem. 329, 278. This journal is
also called Hoppe-Seyler's Zeitschrift Fur Phyiologische Chemie.
"Aminosaure-p-nitroanilide als substrate fur aminopeptidasen und andere
proteolytische Fermente"
2. Novabiochem catalog 97/98 pp311 has a lot of more recent references
on this subject.
Good luck on your synthesis,
Hong Li
Department of Molecular Pharmacology
Albert Einstein College of Medicine
Bronx, NY 10461
>
>This forum has been so helpful in the past, I wish to spring two more
>questions regarding peptide synthesis:
>
>1. A client wishes to couple his active azide-compound to a completed
>peptide which is still on-resin. The Fmoc is the only group removed,
the
>peptidyl- HMP resin (ABI) is dried and in a flask, ready to go.
>Apparently, the azide can only be solubilized effectively in neat
DMSO. I
>wonder about the ability of DMSO to effectively swell the resin - if
anyone
>can enlighten me I would be grateful.
>
>2. Another problem: I have done a little poking around, but have come
up
>empty-handed with regard to protocol for producing p-nitroaniline
amides at
>the COOH-terminus of peptides (pNA peptides). This seems to be used in
>proteolytic assays, where the release of the p-nitroaniline can be
followed
>conveniently using visible wavelengths. Anyone able to point the way?
>
>Thanks for any help,
>
>Alan
>
>=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
>Alan Mahrenholz, Ph.D.
>Dept. Biochemistry
>1153 S. University St.
>Purdue Unversity
>West Lafayette, IN 47907-1153
>
>voice: (765) 494-6383
>lab: 494-6540
>fax (765) 494-7897
>