Suggestion#1: There may be a change in polymerization of acrylamide if
the gel is too close to a magnetic field. Meaning are my gels too close to
a mass spec, which they are not
Suggestion#2: The environemnt of HIS is neutralizing the side chain of
CYS making it appear to look like a Lys to the enzyme. In addition, this
respondant informs me they have noticed cleavage of Gln and His on the
C-terminal side using trypsin.
That's about all I got. Haven't observed this again so far. I'll
let the bulletin board know if I do and maybe I'll have a better idea then.
Joe
Joseph Fernandez
Associate Director
The Rockefeller university
Protein/DNA Technology Center
1230 York Ave. New York, NY 10021
Phone: (212)-327-8869
FAX : (212)-327-8620
email: fernaj@rockvax.rockefeller.edu
Lab Web Page: http:\\pdtc.rockefeller.edu