I have a student who is new to peptide HPLC. The sequence of the peptide=
is
LARHYINLITRQRY. The solubility of the peptide is low in water, somewhat
better in TFA. The peptide seems not to want to come off in acetonitrile
gradients, at least not as a sharp peak. So far, TEA has not helped.
1. What type of column is likely to give the best results: C-4, C-8, or=
C-18?
2. Is there an advantage to going to 300 =C5 pore size?
3. Are there any modifiers we should be trying? Is there a normal serie=
s
of modifiers that one typically runs through.
4. Is there anything special about this sequence that is likely to lead =
to
problems.
Thanks very much for any imput.
Chris Halkides
Christopher Halkides
Dept. of Chemistry, UNCW
601 S. College Road
Wilmington, NC 28403-3297