EBlot

Lawrence J. Dangott (dangott@chemvx.chem.tamu.edu)
Mon, 13 Apr 1998 17:34:19 -0500

A client has a 100kDa protein that does not electroblot from 7.5%
acrylamide gels using CAPS/MeOH or Towbin/MeOH buffers. This is a selective
phenomenon as all other proteins in his sample as well as ~100kDa standards
are successfully blotted. We have reduced methanol concentration and
included low concentrations of SDS without success. Longer blotting times
are also of little avail.

Any suggestions out there?

Thanks,

Larry Dangott

Larry Dangott, Ph.D.
Protein Chemistry Laboratory
Texas A&M University
College Station, TX 77843-3255
(409) 845-2965