O-18 water?...

David.Andrews@arcb.us.astra.com
Wed, 15 Apr 1998 18:08:21 +0200

In a recent paper, the authors used O-18 water in their enzymatic
hydrolysis, in order to label the c-terminal carboxylic acid. Using this
technique, followed by LC/MS/MS, they noted that the b-ions are easy to
identify and discriminate from the y-ions, which can be a big help.
Unfortunately, they say they bought O-18 water from "Miles
Laboratories," but we have no catalog from Miles, and just cold-calling
them wasn't too fruitful, as they are a big company, and one part
doesn't seem to know what the other part is up to. So, I am hoping some
ABRF person has the appropriate number to call, and the appropriate name
for this stuff. Deuterated water is "deuterium oxide," but what do you
call O-18 water?

The paper is "De Novo peptide sequencing in an ion trap mass
spectrometer with 18-O labeling" by Jun Qin, C. J. Herring, and Xiaolong
Zhang
in Rapid Communications in Mass Spectrometry 12, 209-216 (1998).

Thanks!

David W. Andrews, Ph.D.
Principal Scientist, Bioanalytical Group
Astra Research Center Boston
128 Sidney Street
Cambridge, MA 02139

617-234-2608 (voice)
617-576-3030 (fax)
e-mail: david.andrews@arcb.us.astra.com