O-18 water

Dr. Ming F. Tam (MBR405@ccvax.sinica.edu.tw)
Thu, 16 Apr 1998 11:05:50 +0800

Dr. Lehmann published a paper in 1996 (Electrophoresis, 17, 945-953) using
O-18 water in enzymatic digestion. He obtained his O-18 water from:

Enritec
The Weizmann Institute Campus
P.O. Box 2449
REHOVOT 76123
Israel

Tel. 9728936 - 1533
Fax 9728936 - 1532

another supplier for 18-O enriched water is

Cambridge Isotope Laboratories
50 Frontage Road
ANDOVER, Massachusetts 01810-5413
USA

We ordered a batch of O-18 water from Sigma (catalog # 32,987-8) in 1996.
But we couldn't find it in the 1998 catalog any more.

Ming F. Tam

>David W. Andrews wrote:

>In a recent paper, the authors used O-18 water in their enzymatic
>hydrolysis, in order to label the c-terminal carboxylic acid. Using this
>technique, followed by LC/MS/MS, they noted that the b-ions are easy to
>identify and discriminate from the y-ions, which can be a big help.
>Unfortunately, they say they bought O-18 water from "Miles
>Laboratories," but we have no catalog from Miles, and just cold-calling
>them wasn't too fruitful, as they are a big company, and one part
>doesn't seem to know what the other part is up to. So, I am hoping some>
>ABRF person has the appropriate number to call, and the appropriate n>ame
>for this stuff. Deuterated water is "deuterium oxide," but what do yo>u
>call O-18 water>?

>The paper is "De Novo peptide sequencing in an ion trap mass
>spectrometer with 18-O labeling" by Jun Qin, C. J. Herring, and Xiaolong
>Zhang
>in Rapid Communications in Mass Spectrometry 12, 209-216 (1998).

>Thanks!

*********************************
Dr. Ming F. Tam
Institute of Molecular Biology
Academia Sinica
Nankang, Taipei 11529
Taiwan, ROC
mbr405@ccvax.sinica.edu.tw
*********************************