Asp-N Cleavage

Adams, Greg (GAdams@mabioservices.com)
Wed, 22 Apr 1998 14:39:12 -0400

Hi,

I have a protein that I digested with Asp-N (2% w/w for 24 hours). I
suspect that I am also getting some minor cleavage at the N-terminal
side of Glutamic Acid. Has anyone experienced this anomalous cleavage
when using Asp-N.

Greg Adams
MA BioServices