Here is what I have in my notes (from the ABRF BB on MS of MAP
peptides, author is unknown). I have no first hand experience with how
well this works, though.
<fontfamily><param>New_York</param>MS of MAP peptides: wash sample w/
10x with H20 and use as the matrix a saturated solution of
alpha-cyano-4-hydroxycinnamic acid in H2O/CH3CN/TFA
75/25/0.1%</fontfamily>
Dick Cook
>Dear ABRF Colleagues,
>
>I have been asked to analyze (for QC purposes) some MAPS peptides by
MS. I
>have both MALDI and ESI-triple quad MS available. The expected MW of
them
>is around 25kDa.
>
>I seem to recall from the dark recesses of my memory that there are
some
>unique difficulties with this class of peptide. Could anyone with
specific
>experience with mass spectrometry of MAPS peptides share their
experience
>with me?
>
>Regards...Ken
>
>********************************
>
>Ken I. Mitchelhill
>The John Holt Protein Structure Laboratory
>St. Vincent's Institute of Medical Research
>41 Victoria Parade
>Fitzroy 3065 Victoria
>AUSTRALIA
>
>Telephone: 61-3-9288 2480
>Facsimile: 61-3-9416 2676
>
>Email: k.mitchelhill@medicine.unimelb.edu.au
>
>Laboratory:
http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
>ABRF: http://www.medstv.unimelb.edu.au/abrf.html
>
>***********************************