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Ken,
Ruth Angeletti's TIPS article in ABRFnews ("Analysis of MAPS Peptides",
Vol .8, No. 1 page 22) should be helpful....
Clayton
Clayton W. Naeve
Director, Center for Biotechnology
St. Jude Children's Research Hospital
332 N. Lauderdale
Memphis, TN 38105-2794
Tel: (901) 495-3689
Fax: (901) 495-2945
Email: clayton.naeve@stjude.org
> -----Original Message-----
> From: Ken Mitchelhill [SMTP:k.mitchelhill@medicine.unimelb.edu.au]
> Sent: Wednesday, April 22, 1998 8:26 PM
> To: Recipients of ABRF List
> Subject: MAPS peptides by MS
>
> Dear ABRF Colleagues,
>
> I have been asked to analyze (for QC purposes) some MAPS peptides by
> MS. I
> have both MALDI and ESI-triple quad MS available. The expected MW of
> them
> is around 25kDa.
>
> I seem to recall from the dark recesses of my memory that there are
> some
> unique difficulties with this class of peptide. Could anyone with
> specific
> experience with mass spectrometry of MAPS peptides share their
> experience
> with me?
>
> Regards...Ken
>
> ********************************
>
> Ken I. Mitchelhill
> The John Holt Protein Structure Laboratory
> St. Vincent's Institute of Medical Research
> 41 Victoria Parade
> Fitzroy 3065 Victoria
> AUSTRALIA
>
> Telephone: 61-3-9288 2480
> Facsimile: 61-3-9416 2676
>
> Email: k.mitchelhill@medicine.unimelb.edu.au
>
> Laboratory:
> http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHPSL.html
> ABRF: http://www.medstv.unimelb.edu.au/abrf.html
>
> ***********************************
>
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Ken,
Ruth Angeletti's TIPS article in =
ABRFnews ("Analysis of MAPS Peptides", Vol .8, No. 1 page 22) =
should be helpful....
Clayton
Clayton W. Naeve
Director, Center for =
Biotechnology
St. Jude Children's Research =
Hospital
332 N. Lauderdale
Memphis, TN 38105-2794
Tel: (901) 495-3689
Fax: (901) 495-2945
Email: =
clayton.naeve@stjude.org
-----Original Message-----
From: Ken Mitchelhill =
[SMTP:k.mitchelhill@medicine.unimelb.edu.au]
Sent: Wednesday, April 22, 1998 8:26 PM
To: Recipients of ABRF List
Subject: =
MAPS peptides by MS
Dear ABRF =
Colleagues,
I have been asked =
to analyze (for QC purposes) some MAPS peptides by MS. I
have both MALDI and =
ESI-triple quad MS available. The expected MW of them
is around =
25kDa.
I seem to recall =
from the dark recesses of my memory that there are some
unique difficulties =
with this class of peptide. Could anyone with specific
experience with =
mass spectrometry of MAPS peptides share their experience
with me?
Regards...Ken
********************************
Ken I. =
Mitchelhill
The John Holt =
Protein Structure Laboratory
St. Vincent's =
Institute of Medical Research
41 Victoria =
Parade
Fitzroy =
3065 Victoria
AUSTRALIA
Telephone: =
61-3-9288 2480
Facsimile: =
61-3-9416 2676
Email: =
k.mitchelhill@medicine.unimelb.edu.au
Laboratory: http://www.medstv.unimelb.edu.au/WWWDOCS/SVIMRdocs/JHP=
SL.html
ABRF: =
http://www.medstv.unimelb.edu.au/abrf.html<=
/U>
***********************************